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f specific rna coliphage ms2  (ATCC)


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    Structured Review

    ATCC f specific rna coliphage ms2
    F Specific Rna Coliphage Ms2, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 885 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f+specific+rna+coliphage+ms2/Escherichia+coli+bacteriophage+MS2/pmc13095896-63-30-34
    Average 97 stars, based on 885 article reviews
    f specific rna coliphage ms2 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Control:

    Article Title: High-throughput microfluidic quantitative PCR system for the simultaneous detection of antibiotic resistance genes and bacterial and viral pathogens in wastewater.
    Article Snippet: .. Sample process control, DNA/RNA extraction, and reverse transcription (RT) 144 One microliter of a mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American 145 Type Culture Collection, Manassas, VA, USA) and Pseudomonas bacteriophage Φ6 (NBRC 146 105899; National Institute of Technology and Evaluation, Tokyo, Japan) was added to 200 μL 147 Jo urn al Pr e-p roo f 9 each of concentrate and PCR-grade water [i.e., a noninhibitory control (NIC) sample] as 148 molecular process controls (MPCs), according to established recommendations (Haramoto et 149 al., 2018). .. Subsequently, DNA/RNA was extracted from a 200 μL of the concentrated sample 150 using the AllPrep Power Viral DNA/RNA Kit (QIAGEN, Hilden, Germany) on a QIAcube 151 platform (QIAGEN), following the manufacturer’s protocol, and a 100 μL DNA/RNA extract 152 was obtained.

    Article Title: Viral Contaminants in a Philippine Wastewater Treatment Plant: Quantification and Treatment Reduction.
    Article Snippet: .. During extraction, to serve as the molecular process controls (MPCs), 1.0 μL of a mixture of Pseudomonas bacteriophage Φ6 (NBRC 105899; National Institute of Technology and Evaluation, Tokyo, Japan) and F-specific RNA coliphage MS2 (ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) were added to viral concentrates and PCR-grade water (i.e., a non-inhibitory control sample) (Haramoto et al., 2018). ..

    Article Title: Evaluation of the Enterovirus serotype monitoring approach for wastewater surveillance of hand foot and mouth disease using secondary epidemiological surveillance data.
    Article Snippet: .. As a routine each sample was seeded with molecular process control, including 1 μL of the mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) and Pseudomonas bacteriophage Φ6 (NBRC 105899; National Institute of Technology and Evaluation, Tokyo, Japan) (Haramoto et al., 2018). ..

    Article Title: Optimization of a 6-plex Crystal Digital PCR® assay and its application to simultaneous surveillance of enteric and respiratory viruses in wastewater.
    Article Snippet: .. To check for any inhibition in the samples during extraction, RT, and cdPCR, a mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) and Pseudomonas bacteriophage Phi6 (NBRC 105899; National Institute of Technology and Evaluation, Tokyo, Japan) was added as a molecular process control to the virus concentrate and PCR-grade water (i.e., a noninhibitory control sample), as recommended in a previous study (Haramoto et al., 2018). ..

    Article Title: Occurrence and reduction of gastrointestinal pathogens at wastewater treatment facilities in West Java, Indonesia
    Article Snippet: .. F-specific RNA coliphage MS2 (1 μL; ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) was added to 200 μL each of the microbial concentrate and PCR-grade water (a non-inhibitory control [NIC] sample) as a molecular process control (Haramoto et al., 2018; Shrestha et al., 2024). ..

    Article Title: Assessment of environmental factors influencing SARS-CoV-2 in Vietnam's surface water across two years of clinical data.
    Article Snippet: .. As part of establishing molecular process controls, 1.0 μL of a combined viral control mixture comprising F-specific RNA coliphage MS2 (ATCC 15597-B1, American Type Culture Collection, Manassas, VA, USA) and Pseudomonas bacteriophage Phi6 (NBRC 105899, National Institute of Technology and Evaluation, Tokyo, Japan) was spiked in 200 μL of each of the virus concentrate and molecular-grade water. ..

    Article Title: Beyond COVID-19: Wastewater-based epidemiology for multipathogen surveillance and normalization strategies.
    Article Snippet: The resulting pellets in both tubes were resuspended in 800 μL autoclaved MilliQ water (Merck, Rahway, NJ, USA) per tube, thoroughly mixed, and stored as a virus concentrate. .. As recommended previously (Haramoto et al., 2018), in 140 μL each of the virus concentrate and polymerase chain reaction (PCR)-grade water [i.e., a non-inhibitory control (NIC) sample], 1 μL of a mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) and Pseudomonas phage Φ6 (NBRC 105899; National Institute of Technology and Evaluation, Tokyo, Japan) was added as molecular process controls (MPCs). .. RNA was extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany) on a QIAcube platform (QIAGEN), following the manufacturer's protocol.

    Extraction:

    Article Title: High-throughput microfluidic quantitative PCR system for the simultaneous detection of antibiotic resistance genes and bacterial and viral pathogens in wastewater.
    Article Snippet: .. Sample process control, DNA/RNA extraction, and reverse transcription (RT) 144 One microliter of a mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American 145 Type Culture Collection, Manassas, VA, USA) and Pseudomonas bacteriophage Φ6 (NBRC 146 105899; National Institute of Technology and Evaluation, Tokyo, Japan) was added to 200 μL 147 Jo urn al Pr e-p roo f 9 each of concentrate and PCR-grade water [i.e., a noninhibitory control (NIC) sample] as 148 molecular process controls (MPCs), according to established recommendations (Haramoto et 149 al., 2018). .. Subsequently, DNA/RNA was extracted from a 200 μL of the concentrated sample 150 using the AllPrep Power Viral DNA/RNA Kit (QIAGEN, Hilden, Germany) on a QIAcube 151 platform (QIAGEN), following the manufacturer’s protocol, and a 100 μL DNA/RNA extract 152 was obtained.

    Article Title: Viral Contaminants in a Philippine Wastewater Treatment Plant: Quantification and Treatment Reduction.
    Article Snippet: .. During extraction, to serve as the molecular process controls (MPCs), 1.0 μL of a mixture of Pseudomonas bacteriophage Φ6 (NBRC 105899; National Institute of Technology and Evaluation, Tokyo, Japan) and F-specific RNA coliphage MS2 (ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) were added to viral concentrates and PCR-grade water (i.e., a non-inhibitory control sample) (Haramoto et al., 2018). ..

    Article Title: Optimization of a 6-plex Crystal Digital PCR® assay and its application to simultaneous surveillance of enteric and respiratory viruses in wastewater.
    Article Snippet: .. To check for any inhibition in the samples during extraction, RT, and cdPCR, a mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) and Pseudomonas bacteriophage Phi6 (NBRC 105899; National Institute of Technology and Evaluation, Tokyo, Japan) was added as a molecular process control to the virus concentrate and PCR-grade water (i.e., a noninhibitory control sample), as recommended in a previous study (Haramoto et al., 2018). ..

    Reverse Transcription:

    Article Title: High-throughput microfluidic quantitative PCR system for the simultaneous detection of antibiotic resistance genes and bacterial and viral pathogens in wastewater.
    Article Snippet: .. Sample process control, DNA/RNA extraction, and reverse transcription (RT) 144 One microliter of a mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American 145 Type Culture Collection, Manassas, VA, USA) and Pseudomonas bacteriophage Φ6 (NBRC 146 105899; National Institute of Technology and Evaluation, Tokyo, Japan) was added to 200 μL 147 Jo urn al Pr e-p roo f 9 each of concentrate and PCR-grade water [i.e., a noninhibitory control (NIC) sample] as 148 molecular process controls (MPCs), according to established recommendations (Haramoto et 149 al., 2018). .. Subsequently, DNA/RNA was extracted from a 200 μL of the concentrated sample 150 using the AllPrep Power Viral DNA/RNA Kit (QIAGEN, Hilden, Germany) on a QIAcube 151 platform (QIAGEN), following the manufacturer’s protocol, and a 100 μL DNA/RNA extract 152 was obtained.

    Polymerase Chain Reaction:

    Article Title: High-throughput microfluidic quantitative PCR system for the simultaneous detection of antibiotic resistance genes and bacterial and viral pathogens in wastewater.
    Article Snippet: .. Sample process control, DNA/RNA extraction, and reverse transcription (RT) 144 One microliter of a mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American 145 Type Culture Collection, Manassas, VA, USA) and Pseudomonas bacteriophage Φ6 (NBRC 146 105899; National Institute of Technology and Evaluation, Tokyo, Japan) was added to 200 μL 147 Jo urn al Pr e-p roo f 9 each of concentrate and PCR-grade water [i.e., a noninhibitory control (NIC) sample] as 148 molecular process controls (MPCs), according to established recommendations (Haramoto et 149 al., 2018). .. Subsequently, DNA/RNA was extracted from a 200 μL of the concentrated sample 150 using the AllPrep Power Viral DNA/RNA Kit (QIAGEN, Hilden, Germany) on a QIAcube 151 platform (QIAGEN), following the manufacturer’s protocol, and a 100 μL DNA/RNA extract 152 was obtained.

    Article Title: Viral Contaminants in a Philippine Wastewater Treatment Plant: Quantification and Treatment Reduction.
    Article Snippet: .. During extraction, to serve as the molecular process controls (MPCs), 1.0 μL of a mixture of Pseudomonas bacteriophage Φ6 (NBRC 105899; National Institute of Technology and Evaluation, Tokyo, Japan) and F-specific RNA coliphage MS2 (ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) were added to viral concentrates and PCR-grade water (i.e., a non-inhibitory control sample) (Haramoto et al., 2018). ..

    Article Title: Optimization of a 6-plex Crystal Digital PCR® assay and its application to simultaneous surveillance of enteric and respiratory viruses in wastewater.
    Article Snippet: .. To check for any inhibition in the samples during extraction, RT, and cdPCR, a mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) and Pseudomonas bacteriophage Phi6 (NBRC 105899; National Institute of Technology and Evaluation, Tokyo, Japan) was added as a molecular process control to the virus concentrate and PCR-grade water (i.e., a noninhibitory control sample), as recommended in a previous study (Haramoto et al., 2018). ..

    Article Title: Occurrence and reduction of gastrointestinal pathogens at wastewater treatment facilities in West Java, Indonesia
    Article Snippet: .. F-specific RNA coliphage MS2 (1 μL; ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) was added to 200 μL each of the microbial concentrate and PCR-grade water (a non-inhibitory control [NIC] sample) as a molecular process control (Haramoto et al., 2018; Shrestha et al., 2024). ..

    Article Title: Beyond COVID-19: Wastewater-based epidemiology for multipathogen surveillance and normalization strategies.
    Article Snippet: The resulting pellets in both tubes were resuspended in 800 μL autoclaved MilliQ water (Merck, Rahway, NJ, USA) per tube, thoroughly mixed, and stored as a virus concentrate. .. As recommended previously (Haramoto et al., 2018), in 140 μL each of the virus concentrate and polymerase chain reaction (PCR)-grade water [i.e., a non-inhibitory control (NIC) sample], 1 μL of a mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) and Pseudomonas phage Φ6 (NBRC 105899; National Institute of Technology and Evaluation, Tokyo, Japan) was added as molecular process controls (MPCs). .. RNA was extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany) on a QIAcube platform (QIAGEN), following the manufacturer's protocol.

    Inhibition:

    Article Title: Optimization of a 6-plex Crystal Digital PCR® assay and its application to simultaneous surveillance of enteric and respiratory viruses in wastewater.
    Article Snippet: .. To check for any inhibition in the samples during extraction, RT, and cdPCR, a mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) and Pseudomonas bacteriophage Phi6 (NBRC 105899; National Institute of Technology and Evaluation, Tokyo, Japan) was added as a molecular process control to the virus concentrate and PCR-grade water (i.e., a noninhibitory control sample), as recommended in a previous study (Haramoto et al., 2018). ..

    Virus:

    Article Title: Optimization of a 6-plex Crystal Digital PCR® assay and its application to simultaneous surveillance of enteric and respiratory viruses in wastewater.
    Article Snippet: .. To check for any inhibition in the samples during extraction, RT, and cdPCR, a mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) and Pseudomonas bacteriophage Phi6 (NBRC 105899; National Institute of Technology and Evaluation, Tokyo, Japan) was added as a molecular process control to the virus concentrate and PCR-grade water (i.e., a noninhibitory control sample), as recommended in a previous study (Haramoto et al., 2018). ..

    Article Title: Assessment of environmental factors influencing SARS-CoV-2 in Vietnam's surface water across two years of clinical data.
    Article Snippet: .. As part of establishing molecular process controls, 1.0 μL of a combined viral control mixture comprising F-specific RNA coliphage MS2 (ATCC 15597-B1, American Type Culture Collection, Manassas, VA, USA) and Pseudomonas bacteriophage Phi6 (NBRC 105899, National Institute of Technology and Evaluation, Tokyo, Japan) was spiked in 200 μL of each of the virus concentrate and molecular-grade water. ..

    Article Title: Beyond COVID-19: Wastewater-based epidemiology for multipathogen surveillance and normalization strategies.
    Article Snippet: The resulting pellets in both tubes were resuspended in 800 μL autoclaved MilliQ water (Merck, Rahway, NJ, USA) per tube, thoroughly mixed, and stored as a virus concentrate. .. As recommended previously (Haramoto et al., 2018), in 140 μL each of the virus concentrate and polymerase chain reaction (PCR)-grade water [i.e., a non-inhibitory control (NIC) sample], 1 μL of a mixture of F-specific RNA coliphage MS2 (ATCC 15597-B1; American Type Culture Collection, Manassas, VA, USA) and Pseudomonas phage Φ6 (NBRC 105899; National Institute of Technology and Evaluation, Tokyo, Japan) was added as molecular process controls (MPCs). .. RNA was extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany) on a QIAcube platform (QIAGEN), following the manufacturer's protocol.



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    ATCC f specific rna coliphage ms2
    F Specific Rna Coliphage Ms2, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f+specific+rna+coliphage+ms2/Escherichia+coli+bacteriophage+MS2/pmc13095896-63-30-34
    Average 97 stars, based on 1 article reviews
    f specific rna coliphage ms2 - by Bioz Stars, 2026-09
    97/100 stars
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